粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究   总被引：6，自引：0，他引：6  

郝林华  陈靠山  牛德庆  张玉凤 《海洋科学进展》2006,24(1):66-73

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。  相似文献   

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

Influence of large-diameter pipe pile driving on surrounding marine soils and adjacent submarine pipelines     

Jinzhong Dou  Zhong-Jie Zhang 《Marine Georesources & Geotechnology》2021,39(1):15-33

Abstract

With the large-scale development and utilization of ocean resources and space, it is inevitable to encounter existing submarine facilities in pile driving areas, which necessitates a safety assessment. In this article, by referring to a wharf renovation project as a reference, the surrounding soil response and buried pipe deformation during pile driving in a near-shore submarine environment are investigated by three-dimensional (3D) numerical models that consider the pore water effect. Numerical studies are carried out in two different series: one is a case of a single pile focusing on the effect of the minimum plane distance of the pile–pipe, and the other is a case of double piles focusing on the effect of the pile spacing.  相似文献   

山东全新世滨海软土与工程地质灾害的研究   总被引：2，自引：0，他引：2  

吴建政 《海洋地质与第四纪地质》1995,15(3):43-54

山东全新世滨海软土分布在海湾、泻湖、河口区，厚度１－１０ｍ。海湾、泻湖软土为淤泥、淤泥质土，陆源物质供应丰富的浅水区，软土颗粒偏粗，强度相对较高，触变性对建筑物危害性大，陆源物质供应少的深水区，软土颗粒偏细，低强度、高压缩性、沉降变形大和蠕变性是危及建筑物稳定的主要因素。海湾、泻湖软土位于地下水位下，具低透水性，掩埋后短期不易排水固结，软土下为强度较高的冲积物，对一般建筑物，利用强夯、粉喷搅拌桩、  相似文献   

软土海岸地区测量控制点标志的设立     

苏衍坤  李新国  朱绪伟  苏衍国 《海洋测绘》2007,27(3):43-47

分析了软土、湿地、滩涂地区特殊地理环境对测量控制点的影响;主要探讨了此类地区控制点标志的设计、施工。借助于建筑桩基技术和道桥施工方法,首次成功实现了某三角洲湿地滩涂地区统一布网。几年来,结合滩涂湿地测量、海岸防护和滩涂开发工程对部分控制点进行了初步检测,给出了部分检测数据。应用和检测均表明:桩基标石可较好地解决软土、湿地、滩涂地区控制点布设难题,显著提高软土海岸地区测量控制点标志的稳固性,从而延长控制网的可使用期。  相似文献   

种植盐地碱蓬修复滨海盐渍土效果的研究   总被引：25，自引：0，他引：25  

林学政  沈继红  刘克斋  黄晓航 《海洋科学进展》2005,23(1):65-69

利用盐生植物——盐地碱蓬进行天津河口滨海盐碱地的生物修复。通过比较种植盐地碱蓬土壤和对照土壤的电导率、有机质和微生物数量的差异等研究了其修复效果。结果表明，种植区碱蓬根际土壤的电导率与对照土壤相比下降了13％；有机质和总氮与对照土壤相比分别增加43％和18％；根际土壤的微生物数量明显增加，放线菌和真菌分别比对照增加了5倍和16倍。根际微生物优势种群的盐耐受性结果显示，部分根际土壤的优势种群的盐耐受性明显下降，耐盐性较低的微生物种群已成为优势种群。以上结果表明，种植盐地碱蓬对改善滨海盐碱地的生态有明显的效果。  相似文献   

A Study on the Dynamic Analysis of A Tracked Vehicle for Ocean Mining on the Deep Seabed     

Hyung-suk HAN 《中国海洋工程》2003,17(3)

1 .IntroductionOnthedeepseabed ,30 0 0～ 50 0 0mbelowthePacificOcean ,manganesenodulesaresolidifiedandspread ,andmanycountriesintheworldarestudyinghowtominethem .AccordingtoYamazakietal.( 1 998)andAmannetal.( 1 991 ) ,theenvironmentofthedeepseabedisdifferentfromthedrylandinmanyways .Inparticular,thesurfaceoftheseabedisverysoft.Inordertoensuretheperformanceofrunningofamanganesenodulemineronsuchasoftsurface ,thetrackedvehiclemaybeapplied .Ac cordingtoHongandChoi ( 2 0 0 1 ) ,astudyisbeingdone…  相似文献   

复合加载条件下吸力式沉箱基础承载特性数值分析   总被引：2，自引：0，他引：2  

王志云  王栋  栾茂田  范庆来  武科 《海洋工程》2007,25(2):52-56,71

吸力式沉箱基础的承载特性是海洋工程结构设施建造与设计中的一个关键问题。这种新型的深水海洋基础型式,通常承受竖向上拔荷载与水平荷载的共同作用,其工作性能与设计理论远远不能满足工程实践的需要。本文采用有限元分析方法对吸力式沉箱基础的极限承载特性进行数值计算。以大型通用有限元分析软件ABAQUS为平台,通过二次开发,数值实现了Swipe试验加载方法和固定位移比分析方法,针对不同的沉箱长径比、土的强度折减系数,探讨了沉箱基础在垂直上拔荷载和水平荷载单调联合作用下的极限承载力,通过对不同荷载组合的数值计算构造了复合加载条件下沉箱基础破坏包络面。  相似文献   

深层搅拌法在超软弱港口地基上的工程应用     

曹永琅  毛元平  丛建 《海岸工程》2003,22(1):8-13

某港口堆场地基上部5．0m系吹填而成，地表下18m范围均属软土，经真空预压初级加固后地基承载力仅在80—90kPa，局部区域上部淤泥土层土性指标较差，含水量大于50％，地基承载力不足60kPa。为了使地基达到230kPa的承载要求，设计采用深层搅拌法加固超软弱地基。通过现场成桩工艺试验和检测表明，桩身水泥土强度在90d龄期时大于1．80MPa，单桩承载力标准值大于150kN，以φ600桩径、桩长13．5m、置换率为0．308和φ500桩径、桩长13．5m、置换率为0．267两种方案布置的复合地基承载力标准值均超过了230kPa的设计要求。试验结果表明，深层搅拌法在港口超软弱地基土应用只要施工工艺适当，完全可以使地基承载力提高2—3．5倍以土，从而节省大量的工程投资。  相似文献   

非饱和膨胀土边坡的稳定性分析   总被引：2，自引：0，他引：2  

吴珺华  YUAN Jun-ping  袁俊平  卢廷浩 《岩土力学》2008,29(Z1):363-367

为了提高非饱和膨胀土边坡的稳定,采用了土工膜覆盖边坡表面的方法.基于非饱和渗流理论,采用有限元程序对用膜覆盖和不覆盖的边坡分别进行了降雨蒸发干湿循环条件下的数值分析.在计算结果基础上,采用极限平衡法分析了边坡的稳定性.结果表明,用膜覆盖后边坡能长期保持非饱和状态,土体吸力较高,其稳定安全系数明显高于无膜保护的边坡,用复合土工膜覆盖边坡有利于提高非饱和膨胀土边坡的稳定.  相似文献